MicroRNA-17-5p and MicroRNA-130b-3p Promote Radioresistance of Glioma Stem Cells by Targeting PTEN/AKT/HIF-1α Pathway-Controlled Phosphopentose Metabolism

Background. The radioresistance of glioma stem cells (GSCs) is related to some microRNAs (miRs) generated by radiation. This study aimed investigate the effects miR-17-5p and miR-130b-3p on radiosensitivity GSCs. Methods. expressions in SU3 SU3-5R were determined. transfected with or mimics inhibitors used determine cell viability after irradiation as well examine changes supernatant glucose, intracellular glucose 6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate (6-PGDH), reduced nicotinamide adenine dinucleotide phosphate (NADPH), glutathione (GSH), peroxidase (GSH-Px), phosphatase tensin homolog (PTEN), hypoxia-inducible factor-1α (HIF-1α), transporter (GLUT)-1/3, protein kinase B (AKT), p-AKT levels. target gene two miRs was verified luciferase reporter assay. Results. radiation-resistant 8 Gy irradiation-treated high. After transfection mimics, viability, HIF-1α, GLUT-1/3, AKT, expressions, G6PDH, 6-PGDH, NADPH, GSH-Px, GSH levels high, whereas PTEN expression low. opposite also observed miR inhibitors-transfected cells. Further confirmed that could directly bind PTEN. Conclusion. Radiation-induced might cause GSCs, mechanisms associated enhancement antioxidant production, which from increments AKT/HIF-1α signaling pathway-controlled transmembrane transport phosphopentose metabolism targeting